background: current microscopic experimental methods cannot diagnose dna damages present in spermatozoa .therefore, some methods are needed to address the abnormality of the genetic material status on the sperm samples. as reported by many investigators aniline blue staining technique has been used for identifying sperm chromatin condensation. also, chromomycin a3 is used for evaluation of the ...

evidence suggests that human sperm chromatin anomalies adversely affect reproductive outcomes and infertile men possess substantially amount of sperm with chromatin anomalies than fertile men. routine semen analysis evaluates parameters such as sperm motility and morphology, but does not examine the nuclear dna integrity of spermatozoa. it has been suggested that altered nuclear chromatin struc...

Evidence suggests that human sperm chromatin anomalies adversely affect reproductive outcomes and infertile men possess substantially amount of sperm with chromatin anomalies than fertile men. Routine semen analysis evaluates parameters such as sperm motility and morphology, but does not examine the nuclear DNA integrity of spermatozoa. It has been suggested that altered nuclear chromatin struc...

background: this case-control study was designed with the aim of evaluating the role of sperm, oxidative stress and dna damage in idiopathic recurrent pregnancy loss (irpl). this pilot study is the first study done on the indian population which reports the association between dfi, tac and ros in couples experiencing irsa. methods: twenty infertile men with a history of irpl and 20 fertile cont...

The centromere is an essential chromatin domain required for kinetochore recruitment and chromosome segregation in eukaryotes. To perform this role, centro-chromatin adopts a unique structure that provides access to proteins maintains stability under tension during mitosis. This achieved by the presence of nucleosomes containing H3 variant CENP-A, which also acts as epigenetic mark defining cen...